Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

AIMS: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). METHODS AND RESULTS: S. cerevisiae NE095 was prepared by stable i...

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Bibliografiske detaljer
Udgivet i:Biomol Detect Quantif
Main Authors: Da Silva, S.M., Vang, L.K., Olson, N.D., Lund, S.P., Downey, A.S., Kelman, Z., Salit, M.L., Lin, N.J., Morrow, J.B.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2016
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4827694/
https://ncbi.nlm.nih.gov/pubmed/27077050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2016.01.001
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