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A monomer of Pif1 unwinds double-stranded DNA and it is regulated by the nature of the non-translocating strand at the 3′-end
Using a DNA polymerase-coupled assay and FRET-based helicase assays, in this work we show that a monomer of S. cerevisiae Pif1 can unwind double-stranded DNA. The helicase activity of a Pif1 monomer is modulated by the nature of the 3′-ssDNA tail of the substrate and its effect on a Pif1-dependent r...
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| Izdano u: | J Mol Biol |
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| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2016
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4826290/ https://ncbi.nlm.nih.gov/pubmed/26908222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2016.02.017 |
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