Analysis of Monoclonal Antibody Sequence and Post-translational Modifications by Time-controlled Proteolysis and Tandem Mass Spectrometry

Methodology for sequence analysis of ∼150 kDa monoclonal antibodies (mAb), including location of post-translational modifications and disulfide bonds, is described. Limited digestion of fully denatured (reduced and alkylated) antibody was accomplished in seconds by flowing a sample in 8 m urea at a...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Mol Cell Proteomics
Egile Nagusiak: Zhang, Lichao, English, A. Michelle, Bai, Dina L., Ugrin, Scott A., Shabanowitz, Jeffrey, Ross, Mark M., Hunt, Donald F., Wang, Wei-Han
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The American Society for Biochemistry and Molecular Biology 2016
Gaiak:
Technological Innovation and Resources
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4824869/
https://ncbi.nlm.nih.gov/pubmed/26621848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.056721
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