Dual fluorescence detection of protein and RNA in Drosophila tissues

Detection of RNAs by in situ hybridization (ISH) is a well-established technique that permits the study of specific RNA expression patterns in tissues; however, not all tissues are equally amenable to staining using the same procedure. Here we describe a protocol that combines whole-mount immunofluo...

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Udgivet i:Nat Protoc
Main Authors: Toledano, Hila, D’Alterio, Cecilia, Loza-Coll, Mariano, Jones, D Leanne
Format: Artigo
Sprog:Inglês
Udgivet: 2012
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4821427/
https://ncbi.nlm.nih.gov/pubmed/22976352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.105
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