Dynamic Nuclear Polarization of membrane proteins: covalently bound spin-labels at protein-protein interfaces

We demonstrate that dynamic nuclear polarization (DNP) of membrane proteins in lipid bilayers may be achieved using a novel polarizing agent: pairs of spin labels covalently bound to a protein of interest interacting at an intermolecular interaction surface. For gramicidin A, nitroxide tags attached...

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Bibliographic Details
Published in:J Biomol NMR
Main Authors: Wylie, Benjamin J, Dzikovski, Boris G., Pawsey, Shane, Caporini, Marc, Rosay, Melanie, Freed, Jack H., McDermott, Ann E.
Format: Artigo
Language:Inglês
Published: 2015
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4819240/
https://ncbi.nlm.nih.gov/pubmed/25828256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-9919-6
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