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Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications

The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H(2)-based biofuel application as it favours H(2) oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, base...

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Bibliografiske detaljer
Udgivet i:MethodsX
Main Authors: Jugder, Bat-Erdene, Lebhar, Helene, Aguey-Zinsou, Kondo-Francois, Marquis, Christopher P.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4816682/
https://ncbi.nlm.nih.gov/pubmed/27077052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.03.005
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