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Use of carbonate extraction in analyzing moderately hydrophobic transmembrane proteins in the mitochondrial inner membrane

Resistance to sodium carbonate extraction is regarded as a canonical way to distinguish integral membrane proteins (MPs) from other membrane‐associated proteins. However, it has been observed that carbonate extraction releases some mitochondrial integral MPs. Here, by analyzing both artificially des...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Kim, Hayoung, Botelho, Salomé Calado, Park, Kwangjin, Kim, Hyun
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4815233/
https://ncbi.nlm.nih.gov/pubmed/26435163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2817
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