Atypical effect of temperature tuning on the insertion of the catalytic iron−sulfur center in a recombinant [FeFe]‐hydrogenase

The expression of recombinant [FeFe]‐hydrogenases is an important step for the production of large amount of these enzymes for their exploitation in biotechnology and for the characterization of the protein‐metal cofactor interactions. The correct assembly of the organometallic catalytic site, named...

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Bibliographic Details
Published in:Protein Sci
Main Authors: Morra, Simone, Cordara, Alessandro, Gilardi, Gianfranco, Valetti, Francesca
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4815232/
https://ncbi.nlm.nih.gov/pubmed/26362685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2805
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