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Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning

Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target. One potential source of amplification bias is the inherent lack of codon optimization that occurs within phage display libraries. Here we...

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Foilsithe in:Biotechniques
Main Authors: Umlauf, Benjamin J., McGuire, Michael J., Brown, Kathlynn C.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4810453/
https://ncbi.nlm.nih.gov/pubmed/25652031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114256
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