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Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning
Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target. One potential source of amplification bias is the inherent lack of codon optimization that occurs within phage display libraries. Here we...
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| Publicado no: | Biotechniques |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4810453/ https://ncbi.nlm.nih.gov/pubmed/25652031 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114256 |
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