Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses

The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecti...

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Detalhes bibliográficos
Publicado no:Viruses
Main Authors: Yanagisawa, Hironobu, Tomita, Reiko, Katsu, Koji, Uehara, Takuya, Atsumi, Go, Tateda, Chika, Kobayashi, Kappei, Sekine, Ken-Taro
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2016
Assuntos:
Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4810260/
https://ncbi.nlm.nih.gov/pubmed/27072419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v8030070
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