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Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis
We describe a protocol for microinjection of embryos for an emerging model system, the cnidarian sea anemone, Nematostella vectensis. In addition, we provide protocols for carrying out overexpression and knockdown of gene function through microinjection of in vitro–translated mRNAs or gene-specific...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4805419/ https://ncbi.nlm.nih.gov/pubmed/23579781 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.009 |
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