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Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

We describe a protocol for microinjection of embryos for an emerging model system, the cnidarian sea anemone, Nematostella vectensis. In addition, we provide protocols for carrying out overexpression and knockdown of gene function through microinjection of in vitro–translated mRNAs or gene-specific...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Layden, Michael J, Röttinger, Eric, Wolenski, Francis S, Gilmore, Thomas D, Martindale, Mark Q
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4805419/
https://ncbi.nlm.nih.gov/pubmed/23579781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.009
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