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Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose
A method is reported to purify Fibulin-1 from human plasma resulting in a 36% recovery. The steps involve removal of the cryoglobulin and the vitamin K dependent proteins followed by polyethylene glycol and ammonium sulfate precipitations, DEAE-Sephadex column chromatography and finally Factor H-Sep...
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| Publicado no: | Protein Expr Purif |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4803571/ https://ncbi.nlm.nih.gov/pubmed/26826315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2016.01.013 |
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