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Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose

A method is reported to purify Fibulin-1 from human plasma resulting in a 36% recovery. The steps involve removal of the cryoglobulin and the vitamin K dependent proteins followed by polyethylene glycol and ammonium sulfate precipitations, DEAE-Sephadex column chromatography and finally Factor H-Sep...

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Bibliographic Details
Published in:Protein Expr Purif
Main Authors: DiScipio, Richard G., Liddington, Robert C., Schraufstatter, Ingrid U.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4803571/
https://ncbi.nlm.nih.gov/pubmed/26826315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2016.01.013
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