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Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: analogy to bacterial conjugation.

As an early stage of plant transformation by Agrobacterium tumefaciens, the Ti plasmid is nicked at the border sequences that delimit the T-DNA. Cleavage results in covalent attachment of VirD2 to the 5' terminal of the nicked strand by a process resembling initiation of DNA transfer that occur...

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Bibliographic Details
Main Authors: Pansegrau, W, Schoumacher, F, Hohn, B, Lanka, E
Format: Artigo
Language:Inglês
Published: 1993
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC48019/
https://ncbi.nlm.nih.gov/pubmed/8265585
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