Identification of a cytochrome P450 gene by reverse transcription--PCR using degenerate primers containing inosine.
A cytochrome P450-like gene, tentatively named P450CMEF, was amplified by a mixed oligonucleotide-primed amplification of cDNA from C3H mouse embryo fibroblast cells, designated 10T1/2, that had been treated with 7,12-dimethylbenz[a]anthracene (DMBA) or benz[a]anthracene (BA). A set of inosine-conta...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1993
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48008/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7505439/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.24.11483 |
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