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Identification of a cytochrome P450 gene by reverse transcription--PCR using degenerate primers containing inosine.

A cytochrome P450-like gene, tentatively named P450CMEF, was amplified by a mixed oligonucleotide-primed amplification of cDNA from C3H mouse embryo fibroblast cells, designated 10T1/2, that had been treated with 7,12-dimethylbenz[a]anthracene (DMBA) or benz[a]anthracene (BA). A set of inosine-conta...

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Détails bibliographiques
Auteurs principaux: Shen, Z, Wells, R L, Liu, J, Elkind, M M
Format: Artigo
Langue:Inglês
Publié: 1993
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC48008/
https://ncbi.nlm.nih.gov/pubmed/7505439
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