Structurally engineered cytochromes with unusual ligand-binding properties: expression of Saccharomyces cerevisiae Met-80-->Ala iso-1-cytochrome c.

A strategy has been developed to express and purify a recombinant, nonfunctional axial-ligand mutant of iso-1-cytochrome c (Met-80-->Ala) in Saccharomyces cerevisiae in quantities necessary for extensive biophysical characterization. It involves coexpressing in the same plasmid (YEp213) the nonfunct...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Lu, Y, Casimiro, D R, Bren, K L, Richards, J H, Gray, H B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48002/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8265573/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.24.11456
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!