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Fluorescent RNA aptamers as a tool to study RNA-modifying enzymes
RNA-modifying enzymes are difficult to assay due to the absence of fluorometric substrates. Here we show that the Broccoli, a previously reported fluorescent RNA-dye complex, can be modified to contain N(6)-methyladenosine, a prevalent mRNA base modification. Methylated Broccoli is nonfluorescent, b...
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| Udgivet i: | Cell Chem Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4800036/ https://ncbi.nlm.nih.gov/pubmed/26877022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2015.11.018 |
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