The effect of cellular isolation and cryopreservation on the expression of markers identifying subsets of regulatory T cells

BACKGROUND: The role of CD4(+) regulatory T cells (T(regs)) and their subsets during HIV infection is controversial. Cryopreserved peripheral blood mononuclear cells (PBMC) are an important source for assessing number and function of T(regs). However, it is unknown if PBMC isolation and cryopreserva...

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Gepubliceerd in:J Immunol Methods
Hoofdauteurs: Zhang, Weiying, Nilles, Tricia L., Johnson, Jacquett R., Margolick, Joseph B.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4792753/
https://ncbi.nlm.nih.gov/pubmed/26855370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2016.02.004
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