Individual subunits of bacterial luciferase are molten globules and interact with molecular chaperones.

We have studied the assembly of a large heterodimeric protein, bacterial luciferase, by mixing purified subunits expressed separately in bacteria. The individual subunits alpha and beta contain much (66% and 50%, respectively) of the alpha-helix content of the native heterodimer as measured by circu...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Flynn, G C, Beckers, C J, Baase, W A, Dahlquist, F W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47871/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7902573/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.22.10826
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