Effect of enzymatic and mechanical methods of dissociation on neural progenitor cells derived from induced pluripotent stem cells

PURPOSE: To determine the most effective method of dissociating neural stem and progenitor cells into a single-cell suspension. MATERIALS/METHODS: Induced pluripotent stem cells were differentiated toward the neural fate for 4 weeks before clusters were subjected to enzymatic (Accutase, trypsin, Try...

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Udgivet i:Adv Med Sci
Main Authors: Jager, Lindsey D., Canda, Claire-Marie A., Hall, Crystal A., Heilingoetter, Cassandra L., Huynh, Joann, Kwok, Susanna S., Kwon, Jin H., Richie, Jacob R., Jensen, Matthew B.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4785092/
https://ncbi.nlm.nih.gov/pubmed/26523795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.advms.2015.09.005
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