Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

BACKGROUND: Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharom...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Genome Biol
Päätekijät: Smith, Justin D., Suresh, Sundari, Schlecht, Ulrich, Wu, Manhong, Wagih, Omar, Peltz, Gary, Davis, Ronald W., Steinmetz, Lars M., Parts, Leopold, St.Onge, Robert P.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BioMed Central 2016
Aiheet:
Research
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4784398/
https://ncbi.nlm.nih.gov/pubmed/26956608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0900-9
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