Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

BACKGROUND: Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharom...

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Bibliografiske detaljer
Udgivet i:Genome Biol
Main Authors: Smith, Justin D., Suresh, Sundari, Schlecht, Ulrich, Wu, Manhong, Wagih, Omar, Peltz, Gary, Davis, Ronald W., Steinmetz, Lars M., Parts, Leopold, St.Onge, Robert P.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4784398/
https://ncbi.nlm.nih.gov/pubmed/26956608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0900-9
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