Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9

Real-time quantitative PCR (qPCR) protocols specific to the reductive dehalogenase (RDase) genes vcrA, bvcA, and tceA are commonly used to quantify Dehalococcoides spp. in groundwater from chlorinated solvent-contaminated sites. In this study, loop-mediated isothermal amplification (LAMP) was develo...

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Udgivet i:Appl Environ Microbiol
Main Authors: Kanitkar, Yogendra H., Stedtfeld, Robert D., Steffan, Robert J., Hashsham, Syed A., Cupples, Alison M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2016
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4784023/
https://ncbi.nlm.nih.gov/pubmed/26746711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03660-15
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