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Detecting KaiC phosphorylation rhythms of the cyanobacterial circadian oscillator in vitro and in vivo

The central oscillator of the cyanobacterial circadian clock is unique in the biochemical simplicity of its components and the robustness of the oscillation. The oscillator is composed of three cyanobacterial proteins, KaiA, KaiB, and KaiC. If very pure preparations of these three proteins are mixed...

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Dettagli Bibliografici
Pubblicato in:Methods Enzymol
Autori principali: Kim, Yong-Ick, Boyd, Joseph S., Espinosa, Javier, Golden, Susan S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4782789/
https://ncbi.nlm.nih.gov/pubmed/25662456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.10.003
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