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Gene disruption in Salmonella typhimurim by modified λ Red disruption system

There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEing PCR method and the λ Red disruption system were used to disrupt phoP gene in wild type and standard strains of Salmonella typhimurium. Th...

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Detalhes bibliográficos
Publicado no:Iran J Vet Res
Main Authors: Ahani Azari, A, Zahraei Salehi, T, Nayeri Fasaei, B, Alebouyeh, M
Formato: Artigo
Idioma:Inglês
Publicado em: School of Veterinary Medicine, University of Shiraz 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4782703/
https://ncbi.nlm.nih.gov/pubmed/27175194
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