Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae

The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked...

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Bibliografiske detaljer
Udgivet i:Iran J Vet Res
Main Authors: Bu, R. E, Wang, J. L, DebRoy, C, Wu, J. H, Xi, L. G. W, Liu, Y, Shen, Z. Q
Format: Artigo
Sprog:Inglês
Udgivet: School of Veterinary Medicine, University of Shiraz 2015
Fag:
Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4782699/
https://ncbi.nlm.nih.gov/pubmed/27175190
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