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Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae
The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked...
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| Publicado no: | Iran J Vet Res |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
School of Veterinary Medicine, University of Shiraz
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4782699/ https://ncbi.nlm.nih.gov/pubmed/27175190 |
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