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A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Immunophenotyping of peripheral blood by flow cytometry determines changes in the frequency and activation status of peripheral leukocytes during disease and treatment. It has the potential to predict therapeutic efficacy and identify novel therapeutic targets. Whole blood staining utilizes unmanipu...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Koguchi, Yoshinobu, Gonzalez, Iliana L., Meeuwsen, Tanisha L., Miller, William L., Haley, Daniel P., Tanibata-Branham, Alice N., Bahjat, Keith S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4781742/
https://ncbi.nlm.nih.gov/pubmed/26890325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53485
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