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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Reverse genetics, an approach to rescue infectious virus entirely from a cloned cDNA, has revolutionized the field of positive-strand RNA viruses, whose genomes have the same polarity as cellular mRNA. The cDNA-based reverse genetics system is a seminal method that enables direct manipulation of the...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Yun, Sang-Im, Song, Byung-Hak, Kim, Jin-Kyoung, Lee, Young-Min
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780872/
https://ncbi.nlm.nih.gov/pubmed/26780115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53164
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