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Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Three-dimensional (3D) tumor spheroids are utilized in cancer research as a more accurate model of the in vivo tumor microenvironment, compared to traditional two-dimensional (2D) cell culture. The spheroid model is able to mimic the effects of cell-cell interaction, hypoxia and nutrient deprivation...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Beaumont, Kimberley A., Anfosso, Andrea, Ahmed, Farzana, Weninger, Wolfgang, Haass, Nikolas K.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780858/
https://ncbi.nlm.nih.gov/pubmed/26779761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53486
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