A covalent and cleavable antibody-DNA conjugation strategy for sensitive protein detection via immuno-PCR

Immuno-PCR combines specific antibody-based protein detection with the sensitivity of PCR-based quantification through the use of antibody-DNA conjugates. The production of such conjugates depends on the availability of quick and efficient conjugation strategies for the two biomolecules. Here, we pr...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: van Buggenum, Jessie A. G. L., Gerlach, Jan P., Eising, Selma, Schoonen, Lise, van Eijl, Roderick A. P. M., Tanis, Sabine E. J., Hogeweg, Mark, Hubner, Nina C., van Hest, Jan C., Bonger, Kimberly M., Mulder, Klaas W.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780193/
https://ncbi.nlm.nih.gov/pubmed/26947912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep22675
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