A covalent and cleavable antibody-DNA conjugation strategy for sensitive protein detection via immuno-PCR

Immuno-PCR combines specific antibody-based protein detection with the sensitivity of PCR-based quantification through the use of antibody-DNA conjugates. The production of such conjugates depends on the availability of quick and efficient conjugation strategies for the two biomolecules. Here, we pr...

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Udgivet i:Sci Rep
Main Authors: van Buggenum, Jessie A. G. L., Gerlach, Jan P., Eising, Selma, Schoonen, Lise, van Eijl, Roderick A. P. M., Tanis, Sabine E. J., Hogeweg, Mark, Hubner, Nina C., van Hest, Jan C., Bonger, Kimberly M., Mulder, Klaas W.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780193/
https://ncbi.nlm.nih.gov/pubmed/26947912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep22675
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