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Smash and DASH with Cas9
DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
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Published in: | Genome Biol |
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Main Authors: | , |
Format: | Artigo |
Language: | Inglês |
Published: |
BioMed Central
2016
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4779193/ https://ncbi.nlm.nih.gov/pubmed/26944856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0905-4 |
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