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Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA. To examine the most ac...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Nakayama, Yuki, Yamaguchi, Hiromi, Einaga, Naoki, Esumi, Mariko
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4777359/
https://ncbi.nlm.nih.gov/pubmed/26937682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0150528
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