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Rapid Synthesis of a Long Double-Stranded Oligonucleotide from a Single-Stranded Nucleotide Using Magnetic Beads and an Oligo Library

Chemical synthesis of oligonucleotides is a widely used tool in the field of biochemistry. Several methods for gene synthesis have been introduced in the growing area of genomics. In this paper, a novel method of constructing dsDNA is proposed. Short (28-mer) oligo fragments from a library were asse...

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Publicat a:PLoS One
Autors principals: Pengpumkiat, Sumate, Koesdjojo, Myra, Rowley, Erik R., Mockler, Todd C., Remcho, Vincent T.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4773231/
https://ncbi.nlm.nih.gov/pubmed/26930667
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149774
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