Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves

The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysi...

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Veröffentlicht in:PLoS One
Hauptverfasser: Feng, Xia, Ma, Jun-Wu, Sun, Shi-Qi, Guo, Hui-Chen, Yang, Ya-Min, Jin, Ye, Zhou, Guang-Qing, He, Ji-Jun, Guo, Jian-Hong, Qi, Shu-yun, Lin, Mi, Cai, Hu, Liu, Xiang-Tao
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2016
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4773165/
https://ncbi.nlm.nih.gov/pubmed/26930597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149569
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