Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves

The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysi...

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Bibliographic Details
Published in:	PLoS One
Main Authors:	Feng, Xia, Ma, Jun-Wu, Sun, Shi-Qi, Guo, Hui-Chen, Yang, Ya-Min, Jin, Ye, Zhou, Guang-Qing, He, Ji-Jun, Guo, Jian-Hong, Qi, Shu-yun, Lin, Mi, Cai, Hu, Liu, Xiang-Tao
Format:	Artigo
Language:	Inglês
Published:	Public Library of Science 2016
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC4773165/ https://ncbi.nlm.nih.gov/pubmed/26930597 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149569
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Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves

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