Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates

Herein we describe a protocol that uses hollow-fiber flow field-flow fractionation (FFF) coupled with multiangle light scattering (MALS) for hydrodynamic size-based separation and characterization of complex protein aggregates. The fractionation method, which requires 1.5 h to run, was successfully...

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Dettagli Bibliografici
Pubblicato in:Nat Protoc
Autori principali: Tanase, Maya, Zolla, Valerio, Clement, Cristina C, Borghi, Francesco, Urbanska, Aleksandra M, Rodriguez-Navarro, Jose Antonio, Roda, Barbara, Zattoni, Andrea, Reschiglian, Pierluigi, Cuervo, Ana Maria, Santambrogio, Laura
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4771483/
https://ncbi.nlm.nih.gov/pubmed/25521790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.009
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