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Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain

Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4771324/
https://ncbi.nlm.nih.gov/pubmed/26712540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03603-15
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