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Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions

The use of fluorogenic compounds in cell and molecular biology has increased in both frequency and range of applications. However, such compounds may introduce artifacts in intracellular fluorescence and cell number estimations as a consequence of interaction with exogenous stimulants, necessitating...

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Detalhes bibliográficos
Publicado no:J Biol Methods
Main Authors: Miles, Fayth L., Lynch, Jill E., Sikes, Robert A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4770449/
https://ncbi.nlm.nih.gov/pubmed/26937420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2015.73
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