The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue

Quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) remains the most sensitive technique for nucleic acid quantification. Its popularity is reflected in the remarkable number of publications reporting RT-qPCR data. Careful normalisation within RT-qPCR studies is imperati...

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Detalhes bibliográficos
Publicado no:F1000Res
Main Authors: Dowling, Catríona M., Walsh, Dara, Coffey, John C., Kiely, Patrick A.
Formato: Artigo
Idioma:Inglês
Publicado em: F1000Research 2016
Assuntos:
Research Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4768652/
https://ncbi.nlm.nih.gov/pubmed/26962435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/f1000research.7656.2
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