Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Environmental microbes are a great source of industrially valuable enzymes with potent and unique catalytic activities. Unfortunately, the majority of microbes remain unculturable and thus are not accessible by culture-based methods. Recently, culture-independent metagenomic approaches have been suc...

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Pubblicato in:Sci Rep
Autori principali: Nakamura, Kazuki, Iizuka, Ryo, Nishi, Shinro, Yoshida, Takao, Hatada, Yuji, Takaki, Yoshihiro, Iguchi, Ayaka, Yoon, Dong Hyun, Sekiguchi, Tetsushi, Shoji, Shuichi, Funatsu, Takashi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4768102/
https://ncbi.nlm.nih.gov/pubmed/26915788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep22259
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