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Comparison of fecal pooling methods and DNA extraction kits for the detection of Mycobacterium avium subspecies paratuberculosis

The aim of the study was to develop a sensitive method using quantitative real‐time polymerase chain reaction (qPCR) with pooled fecal samples for the screening of Johne's disease (JD). Manufacturer‐specified and our new pooling method in combination with five commercial kits for DNA extraction...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Microbiologyopen
Päätekijät: Mita, Akiko, Mori, Yasuyuki, Nakagawa, Tetsuo, Tasaki, Tomoko, Utiyama, Katsuo, Mori, Hitomi
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: John Wiley and Sons Inc. 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4767428/
https://ncbi.nlm.nih.gov/pubmed/26666871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mbo3.318
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