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Cloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae

The ftsZ gene from Xanthomonas oryzae pv. Oryzae was amplified by PCR with the specific primers, and the recombinant plasmid pET-22b-ftsZ was constructed successfully. The FtsZ with a 6× His tag was overexpressed in a soluble form in Escherichia coli BL21 and purified through a Ni-NTA agarose column...

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Библиографические подробности
Опубликовано в: :Springerplus
Главные авторы: Dai, Leng, Huang, Yunhong, Chen, Yang, Long, Zhong-er
Формат: Artigo
Язык:Inglês
Опубликовано: Springer International Publishing 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4764595/
https://ncbi.nlm.nih.gov/pubmed/27026842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40064-016-1876-3
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