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A high-throughput method for genotyping S-RNase alleles in apple

We present a new efficient screening tool for detection of S-alleles in apple. The protocol using general and multiplexed primers for PCR reaction and fragment detection on an automatized capillary DNA sequencer exposed a higher number of alleles than any previous studies. Analysis of alleles is mad...

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Détails bibliographiques
Publié dans:Mol Breed
Auteurs principaux: Larsen, Bjarne, Ørgaard, Marian, Toldam-Andersen, Torben Bo, Pedersen, Carsten
Format: Artigo
Langue:Inglês
Publié: Springer Netherlands 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4760992/
https://ncbi.nlm.nih.gov/pubmed/26941563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11032-016-0448-0
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