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Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media – Vitrification versus Slow Freezing Methods

Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: von Bomhard, Achim, Elsässer, Alexander, Ritschl, Lucas Maximilian, Schwarz, Silke, Rotter, Nicole
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758583/
https://ncbi.nlm.nih.gov/pubmed/26890410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149660
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