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Refolding process of cysteine-rich proteins:Chitinase as a model
BACKGROUND: Recombinant proteins overexpressed in E. coli are usually deposited in inclusion bodies. Cysteines in the protein contribute to this process. Inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed...
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| Publicado no: | Rep Biochem Mol Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Varastegan Institute for Medical Sciences
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4757093/ https://ncbi.nlm.nih.gov/pubmed/26989746 |
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