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Functional complementation of an Escherichia coli ribonuclease H mutation by a cloned genomic fragment from the trypanosomatid Crithidia fasciculata.

A gene designated Cfa RNH1 has been cloned by complementation of an RNase H deficiency in an Escherichia coli rnhA mutant by using a genomic DNA library from the trypanosomatid Crithidia fasciculata. The encoded RNase H is predicted to have 494 amino acid residues and a molecular mass of 53.7 kDa. T...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Campbell, A G, Ray, D S
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47565/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8415705/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.20.9350
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