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Selenomethionine, p-Cyanophenylalanine Pairs Provide a Convenient, Sensitive, Non-Perturbing Fluorescent Probe of Local Helical Structure
The use of selenomethionine (M(Se))-p-cyanophenylalanine (F(CN)) pairs to probe protein structure is demonstrated. M(Se) quenches F(CN) fluorescence via electron transfer. Both residues can be incorporated recombinantly or by peptide synthesis. Time-resolved and steady-state fluorescence measurement...
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| Publicado en: | Chem Commun (Camb) |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4755734/ https://ncbi.nlm.nih.gov/pubmed/26686928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5cc08232c |
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