A BioBrick™-Compatible Vector for Allelic Replacement Using the XylE Gene as Selection Marker

BACKGROUND: Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods include high false positive rates due to mutations in counter-selection genes, an...

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Bibliographische Detailangaben
Veröffentlicht in:Biol Proced Online
Hauptverfasser: Casanova, Michela, Pasotti, Lorenzo, Zucca, Susanna, Politi, Nicolò, Massaiu, Ilaria, Calvio, Cinzia, Cusella De Angelis, Maria Gabriella, Magni, Paolo
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2016
Schlagworte:
Short Report
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4752771/
https://ncbi.nlm.nih.gov/pubmed/26877712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-016-0036-z
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