Purification and characterization of recombinant G16 alpha from Sf9 cells: activation of purified phospholipase C isozymes by G-protein alpha subunits.

A cDNA encoding G16 alpha, the alpha subunit of a heterotrimeric guanine nucleotide-binding protein, was expressed in Sf9 cells using recombinant baculovirus. G16 alpha in membrane extracts of Sf9 cells activated phospholipase C-beta 1 (PLC-beta 1) in the presence of guanosine 5'-[gamma-thio]triphos...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Kozasa, T, Hepler, J R, Smrcka, A V, Simon, M I, Rhee, S G, Sternweis, P C, Gilman, A G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1993
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47525/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8415674/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.19.9176
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