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Utility of 5-Cyanotryptophan Fluorescence as a Sensitive Probe of Protein Hydration

Tryptophan (Trp) fluorescence has been widely used to interrogate the structure, dynamics and function of proteins. In particular, it provides a convenient and site-specific means to probe a protein’s hydration status and dynamics. Herein, we show that a tryptophan analog, 5-cyanotryptophan (Trp(CN)...

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Bibliografiske detaljer
Udgivet i:J Phys Chem B
Main Authors: Markiewicz, Beatrice N., Mukherjee, Debopreeti, Troxler, Thomas, Gai, Feng
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4752393/
https://ncbi.nlm.nih.gov/pubmed/26783936
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.5b12233
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