A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transm...

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Udgivet i:eLife
Main Authors: Shahidi, Réza, Williams, Elizabeth A, Conzelmann, Markus, Asadulina, Albina, Verasztó, Csaba, Jasek, Sanja, Bezares-Calderón, Luis A, Jékely, Gáspár
Format: Artigo
Sprog:Inglês
Udgivet: eLife Sciences Publications, Ltd 2015
Fag:
Neuroscience
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4749568/
https://ncbi.nlm.nih.gov/pubmed/26670546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.11147
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