Cloning, expression, purification and characterization of lipase from Bacillus licheniformis, isolated from hot spring of Himachal Pradesh, India

In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusio...

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Bibliographische Detailangaben
Veröffentlicht in:3 Biotech
Hauptverfasser: Kaur, Gagandeep, Singh, Amninder, Sharma, Rohit, Sharma, Vinay, Verma, Swati, Sharma, Pushpender K.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer Berlin Heidelberg 2016
Schlagworte:
Original Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4746201/
https://ncbi.nlm.nih.gov/pubmed/28330118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-016-0369-y
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