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Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

[Image: see text] A fluorescent reagentless biosensor for inorganic phosphate (P(i)), based on the E. coli PstS phosphate binding protein, was redesigned to allow measurements of higher P(i) concentrations and at low, substoichiometric concentrations of biosensor. This was achieved by weakening P(i)...

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Dettagli Bibliografici
Pubblicato in:Biochemistry
Autori principali: Solscheid, Claudia, Kunzelmann, Simone, Davis, Colin T., Hunter, Jackie L., Nofer, Annie, Webb, Martin R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2015
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4741387/
https://ncbi.nlm.nih.gov/pubmed/26199994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00449
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