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Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

[Image: see text] A fluorescent reagentless biosensor for inorganic phosphate (P(i)), based on the E. coli PstS phosphate binding protein, was redesigned to allow measurements of higher P(i) concentrations and at low, substoichiometric concentrations of biosensor. This was achieved by weakening P(i)...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Solscheid, Claudia, Kunzelmann, Simone, Davis, Colin T., Hunter, Jackie L., Nofer, Annie, Webb, Martin R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2015
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4741387/
https://ncbi.nlm.nih.gov/pubmed/26199994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00449
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